Title
Some beneficial effects of vanillin on the behavior in Parkinson’s disease model
Poster
Description
Andrey Popatanasov; Velichka Popatanasova
Institute of Neurobiology, Bulgarian Academy of Sciences, Sofia
Institute of Neurobiology, Bulgarian Academy of Sciences, Sofia
Subject
Vanillin is compound found in the flowers and fruits of many species from Orchidaceae
family and is often used in human diet as flavoring agent. Vanillin is known to have also
beneficial action in various pathologies as cancer, metabolite disturbances, intoxications,
etc. Parkinson’s disease (PD) is neurological disease with specific motor and cognitive
disturbances and is associated high oxidative stress and mitochondrial dysfunction. Scope
of the study: To evaluate some effects of vanillin upon the behavior in rats with model of
PD induced with hemistriatal injection of 6-hydroxydopamine (6-OHDA). Methods: The PD
model was produced via right striatal 6-OHDA (2 µg in 2 µl saline) injection of male Wistar
rats. Vanillin treatment was with dose 50 mg/kg i.p. for 5 days. Vanillin effects were
evaluated via behavioral tests- learning and memory (Passive avoidance test), motor
coordination (Rot-a-rod-test). Student t-test was used at p<0.05. Results and discussion:
PD-model was verified by apomorphine test on the 2nd and 3rd week after the operation
and was compared to sham operated animals. There was significant performance increase
in learning and memory processes. Motor coordination in vanillin-treated animals was
improved (both on 2nd and 3rd week) in compare to PD-controls. Vanillin is multiple route
antioxidant. Vanillin as chelator and oxygen radical quencher probably reduces free radical
levels thus minimizing the neuronal and mitochondrial damage in 6-OHDA intoxicated
animals.
family and is often used in human diet as flavoring agent. Vanillin is known to have also
beneficial action in various pathologies as cancer, metabolite disturbances, intoxications,
etc. Parkinson’s disease (PD) is neurological disease with specific motor and cognitive
disturbances and is associated high oxidative stress and mitochondrial dysfunction. Scope
of the study: To evaluate some effects of vanillin upon the behavior in rats with model of
PD induced with hemistriatal injection of 6-hydroxydopamine (6-OHDA). Methods: The PD
model was produced via right striatal 6-OHDA (2 µg in 2 µl saline) injection of male Wistar
rats. Vanillin treatment was with dose 50 mg/kg i.p. for 5 days. Vanillin effects were
evaluated via behavioral tests- learning and memory (Passive avoidance test), motor
coordination (Rot-a-rod-test). Student t-test was used at p<0.05. Results and discussion:
PD-model was verified by apomorphine test on the 2nd and 3rd week after the operation
and was compared to sham operated animals. There was significant performance increase
in learning and memory processes. Motor coordination in vanillin-treated animals was
improved (both on 2nd and 3rd week) in compare to PD-controls. Vanillin is multiple route
antioxidant. Vanillin as chelator and oxygen radical quencher probably reduces free radical
levels thus minimizing the neuronal and mitochondrial damage in 6-OHDA intoxicated
animals.