Title
A study on the effects of Pb, meso-2,3-dimercaptosuccinic acid (DMSA) and salinomycin on the spleen of Pb-intoxicated mice
Poster
Description
Kalina Kamenova(1), Yordanka Gluhcheva(2), Donika Dimova(2), Juliana Ivanova(3)
1-Faculty of Chemistry and Pharmacy, University of Sofia ‘‘St. Kliment Ohridski”
2-Institute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences
3-Faculty of Medicine, University of Sofia ‘‘St. Kliment Ohridski”
2-Institute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences
3-Faculty of Medicine, University of Sofia ‘‘St. Kliment Ohridski”
Subject
Lead (Pb) is a well-known toxic agent. Exposure to Pb through air, food, water, household dust causes adverse effects in humans and animals. The aim of this study was to compare the effects of meso-2,3-dimercaptosuccinic acid (DMSA) and salinomycin on the hematological parameters, serum iron level, TIBC and the endogenous concentrations of Ca, Cu, Fe, Zn in the spleen of Pb-intoxicated mice. Treatment of Pb-intoxicated mice with DMSA or salinomycin did not alter the hematological parameters. Our study demonstrated that Pb(II) intoxication caused a significant decrease in serum Fe concentration but did not affect TIBC compared to the untreated animals. Treatment with DMSA or salinomycin restored the Fe concentration to the normal control values. Pb(II) caused a significant increase of Pb and Ca and decrease of the Cu and Fe concentrations in the spleen compared to the untreated animals. It should be pointed that the treatment of Pb-intoxicated animals with DMSA increased the Cu concentration in spleen compared to the control group. Salinomycin restored the Cu concentration in spleen to normal control values.Treatment with DMSA or salinomycin recovered the Fe concentration in the spleen compared to the toxic group. Salinomycin however increased the Ca concentration in the spleen. Our data demonstrated that Pb can affect spleen function by alterating the homeostasis of biometal ions. DMSA and salinomycin mobilized the toxic metal ion from the spleen. The effect of salinomycin on the Ca endogenous and the effect of DMSA on the Cu endogenous content should be taken into account.